Indah Nur Khulillah, Abdul Latief Abadi, Luqman Qurata Aini


Environmentally friendly and conventional cultivation systems that are selected and used by farmers can directly or indirectly affect microbes in the soil. Microbial population diversity can be used as a sensitive parameter to soil quality. Bacterial populations are a determining factor in ecosystems that are important because of biological and biogeochemical cycles, and heterotrophic activities. This study suggests looking at environmentally friendly (O1) and conventional (K1, K2, K3) soil bacteria on soil planted with sweet maize and function of bacteria in ecosystems. The results of this study that diversity on environmentally friendly was higher than conventional soil. The value was 1,775; while those of the conventional lands 1, 2 and 3 were 1,587; 1,245 and 1,320. In O1 soil, the most common genus was Agrobacterium and bacteria which were only found in environmentally friendly soils were Bacillus and Clostridium. Bacteria found such as O1F and O1G which were related to B. paramycoides could be used as biological agents against Cercospora leaf spot disease, B. megaterium could bind nitrate to the rhizosphere and dissolve phosphate in the soil, and B. aryabhattai which was potential as biological fertilizers and bioremediation


Bacillus; mancozeb; propineb; sweet maize

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Adriano, M.D.L., Gutiérrez, F., Dendooven, L. and Salvador-Figueroa, D. 2012. Influence of compost and liquid bioferment on the chemical and biological characteristics of soil cultivated with banana (Musa spp. L.). Journal of Soil Science and Plant Nutrition 12(1): 33-43.

Ariyanto, S.E. 2011. Perbaikan kualitas pupuk kandang sapi dan aplikasinya pada tanaman jagung manis (Zea mays saccharata Sturt). Jurnal Sains dan Teknologi 4(2): 164-175.

Balouiri, M., Sadiki, M. And Ibnsouda, S.K. 2016. Methods for in vitro evaluating antimicrobial activity: a review. Journal of Pharmaceutical Analysis 6(2): 71-79.

Bargabus, R.L., Zidack, N.K. and Jacobsen, B,J. 2002. Characterisation of systemic resistance in sugar beet elicited by a non-pathogenic, phyllosphere-colonizing Bacillus mycoides, biological control agent. Physiological and Molecular Plant Pathology 61: 289-298.

Bonanomi, G., De Filippis, F., Cesarano, G., La Storia, A., Ercolini, D. and Scala, F. 2016. Organic farming induces changes in soil microbiota that affect agro-ecosystem functions. Soil Biology and Biochemistry 103: 327-336.

Chen, S., Edwards, C.A. and Subler, S. 2001. Effects of the fungicides benomyl, captan and chlorothalonil on soil microbial activity and nitrogen dynamics in laboratory incubations. Soil Biology and Biochemistry 33: 1971-1980.

Chou, Y.M., Shen, F.T., Chiang, S.C. and Chang, C.M. 2017. Functional diversity and dominant populations of bacteria in banana plantation soils as influenced by long-term organic and conventional farming. Applied Soil Ecology 110: 21-33.

Chu, S., Zhang, D., Zhi, Y., Wang, B., Chi, C., Zhang, D., Liu, Y. and Zhou, P. 2018. Enhanced removal of nitrate in the maize rhizosphere by plant growth-promoting Bacillus megaterium NCT-2, and its colonization pattern in response to nitrate. Chemosphere 208: 316-324.

Cyco?, M., Piotrowska-Seget, Z. and Kozdrój, J. 2010. Responses of indigenous microorganisms to a fungicidal mixture of mancozeb and dimethomorph added to sandy soils. Int. Biodeterioration & Biodegradation 64(4): 316-323.

Cycon, M., Markowicz, A. and Piotrowska-seget, Z. 2013. Structural and functional diversity of bacterial community in soil treated with the herbicide napropamide estimated by the DGGE, CLPP and r/K-strategy approaches. Applied Soil Ecology 72: 242-250.

de Vos, P., Garrity, G.M., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.H. and Whitman, W.B. 2009. Bergey's manual of systematic bacteriology (WB Whitman, Ed.). second. Springer, Athens, USA.

Fantke, P., R. Charles, L.F. de Alencastro, R. Friedrich, and O. Jolliet. 2011. Plant uptake of pesticides and human health: dynamic modeling of residues in wheat and ingestion intake. Chemosphere 85(10): 1639-1647.

Fu, L., Ruan, Y., Tao, C., Li, R. And Shen, Q. 2016. Continous application of bioorganic fertilizer induced resilient culturable bacteria community associated with banana Fusarium wilt suppression. Nat. Publ. Gr. (2): 1-11.

Hartmann, M., Fliessbach, A., Oberholzer, H. and Widmer, F. 2006. Ranking the magnitude of crop and farming system effects on soil microbial biomass and genetic structure of bacterial communities. FEMS Microbiology Ecology 57: 378-388.

Hartmann, M., Frey, B., Mayer, J., Mader, P. and Widmer, F. 2015. Distinct soil microbial diversity under long-term organic and conventional farming. ISME Journal 9: 1177-1194.

Hättenschwiler, S., Tiunov, A.V. and Scheu, S. 2005. Biodiversity and litter decomposition in terrestrial ecosystems. Annual Review of Ecology, Evolution, and Systematics 36(1): 191-218.

Hermann, T. 2003. Industrial production of amino acids by coryneform bacteria. Journal of Biotechnology 104: 155-172.

Huang, F., Zhang, Y., Zhang, L., Wang, S., Feng, Y. And Rong, N. 2019. Complete genome sequence of Bacillus megaterium JX285 isolated from Camellia oleifera rhizosphere. Computational Biology and Chemistry 79(11): 1-5.

Indahwati, R. 2012. Pengaruh sistem pertanian ramah lingkungan terhadap kualitas tanah pada lahan apel di kelompok tani Makmur Abadi, Tulungrejo, Kota Batu.

Janse, J.D. 2005. Phytobacteriology?: Principles and Practice. CABI Publishing, Wageningen, Netherlands.

Li, L., Qu, Z., Jia, R., Wang, B., Wang, Y. and Qu., D 2017. Excessive input of phosphorus significantly affects microbial Fe (III) reduction in flooded paddy soils by changing the abundances and community structures of Clostridium and Geobacteraceae. Science of the Total Environment 607-608: 982-991.

Lo, C.-C. 2010. Effect of pesticides on soil microbial community. Journal of Environmental Science and Health Part B 45(5): 348-359.

Ludwig, J.A. and. Reynolds, J.F. 1988. Statistical ecology: Primer on methods and computing. John Wiley and Sons Inc., Canada.

Lupwayi, N.Z., Harker, K.N., Dosdall, L.M., Turkington, T.K., Blackshaw, R.E.,. O'Donovan, J.T., Cárcamo, H.A., Otani, J.K. and Clayton, G.W. 2009. Changes in functional structure of soil bacterial communities due to fungicide and insecticide applications in canola. Agriculture, Ecosystem, and Environment 130(3-4): 109-114.

LV, Q., Chen, C., Xu, Y., Hu, S., Wang, L., Sun, K., Chen, X. and Li, X. 2017. Optimization of Agrobacterium tumefaciens - mediated transformation systems in tea plant (Camellia sinensis). Horticultural Plant Journal 3(3): 105-109.

Meenakshi, S.N., Jeyaramraja, P.R. and Rajesh, M. 2007. Degradation of the fungicides, azoxystrobin and difenoconazole in soil and their influence on soil microbial activity. Pest Technology 1(2): 133-138.

Mendes, L.W., Tsai, S.M., Navarrete, A.A., de Hollander, M., van Veen, J.A. and Kuramae, E.E. 2015. Soil-borne microbiome: linking diversity to function. Microbial Ecology 70(1): 255-265..

Muhammad, A. 2015. Berbisnis Jagung Manis Hasilnya Melangit. Malangtimes. (accessed 8 February 2018).

Nimtz, M., Mort, A., Wray, V., Domke, T., Zhang, Y., Coplin, D.L. and Geider, K. 1996. Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii. Carbohydrate Research 288: 189-201.

Pailan, S., Gupta, D., Apte, S., Krishnamurthi, S. and Saha, P. 2015. Degradation of organophosphate insecticide by a novel Bacillus aryabhattai strain SanPS1, isolated from soil of agricultural field in Burdwan, West Bengal, India. International Biodeterioration and Biodegradation 103: 191-195.

Perez-piqueres, An., Edel-hermann, V., Alabouvette, C. and Steinberg, C. 2006. Response of soil microbial communities to compost amendments. Soil Biology and Biochemistry 38: 460-470.

Ramesh, A., Sharma, S.K., Sharma, M.P., Yadav, N. and Joshi, O.P. 2014. Inoculation of zinc solubilizing Bacillus aryabhattai strains for improved growth, mobilization and biofortification of zinc in soybean and wheat cultivated in Vertisols of central India. Applied Soil Ecology 73: 87-96.

Sang, M.K., and Kim, K.D. 2012. Plant growth-promoting rhizobacteria suppressive to Phytophthora blight affect microbial activities and communities in the rhizosphere of pepper (Capsicum annuum L.) in the field. Applied Soil Ecology 62: 88-97.

Schaad, N.W., Jones, J.B. and Chun, W. 2001. Laboratory guide for identification of plant pathogenic bacteria. APS Press, Minnesota, USA.

Schutte, U.M.E., Abdo, S.Z., Foster, S.J., Ravel, S.J., Bunge, J., Solheim, B. and Forney, L.J. 2010. Bacterial diversity in a glacier foreland of the high Arctic. Molecular Ecology 19: 54-66. d

Shen, Z., Zhong, S., Wang, Y., Wang, B., Mei, X., Li, R., Ruan, Y. And Shen, Q. 2013. European Journal of Soil Biology Induced soil microbial suppression of banana fusarium wilt disease using compost and biofertilizers to improve yield and quality. European Journal of Soil Biology 57: 1-8.

Singh, N., Gupta, S., Marwa, N., Pandey, V., Verma, P.C., Rathaur, S. and Singh, N. 2016. Arsenic mediated modifications in Bacillus aryabhattai and their biotechnological applications for arsenic bioremediation. Chemosphere 164: 524-534.

Smith, K.P. and Goodman, R.M. 1999. Host variations for interactions with beneficial plant-associated microbes. Annual Review of Phytopathology 37: 473-491.

Su?owicz, S. and Piotrowska-Seget, Z. 2016. Response of microbial communities from an apple orchard and grassland soils to the first-time application of the fungicide tetraconazole. Ecotoxicology and Environmental Safety 124: 193-201.

Tchagang, C.F., Xu, R., Overy, D., Blackwell, B., Chabot, D., Hubbard, K. And Tambong, .T. 2018. Diversity of bacteria associated with corn roots inoculated with Canadian woodland soils, and description of Pseudomonas. Heliyon (8): 1-25.

Tomer, V. and Sangha, J.K. 2013. Vegetable processing at household level: effective tool against pesticide residue exposure. Journal of Environmental Science, Toxicology, and Food Technology 6(2): 43-53.

Turnbull, P.C.B., Sirianni, N.M., Lebron, C.I., Samaan, M.N., Sutton, F.N., Reyes, A.E. and L.F.P. Jr. 2004. MICs of selected antibiotics for Bacillus anthracis, Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides from a range of clinical and environmental sources as determined by the Etest. Journal of Clinical Microbiology 42(8): 3626-3634. doi: 10.1128/JCM.42.8.3626.

Voidarou, C., Bezirtzoglou, E., Alexopoulos, A., Plessas, S., Stefanis, C., Papadopoulos, I., Vavias, S., Stavropoulou, E., Fotou, K., Tzora, A. and Skoufos, I. 2011. Occurrence of Clostridium perfringens from different cultivated soils. Anaerobe 17(6): 320-324.

Walia, A., Mehta, P., Guleria, S., Chauhan, A. and Shirkot, C.K. 2014. Impact of fungicide mancozeb at different application rates on soil microbial populations, soil biological processes, and enzyme activities in soil. Science World Journal 4: 1-9.

Wang, H.C., Chen, X.J., Cai, L.T., Cao, Y., Lu, N., Xia, H.Q., Wang, M.S. and. Shang, S.H. 2013. Race distribution and distribution of sensitivities to mefenoxam among isolates of Phytophthora parasitica var. nicotianae in Guizhou province of China. Crop Protection 52: 136-140.

White, D.C. and McNaughton, S.J. 1997. Chemical and molecular approaches for rapid assessment of the biological status of soils. p. 371-396. In Pankhurst, C.E., Doube, B.M., Gupta, V.V.S.R. (eds.), CAB International. CAB International.

Xu, S. 2000. Environmental fate of mancozeb. Sacramento, CA.

Yeates, G.W. and Saggar, S. 1998. Comparison of soil microbial properties and fauna under tussock - grassland and pine plantation. Journal of Royal Society of New Zealand 28(3): 523-535.

Yu, Y., Chu, X., Pang, G., Xiang, Y. and Fang, H. 2009. Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil. Journal of Environmental Science 21(2): 179-185.



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